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Image Search Results
Journal: Advanced Science
Article Title: A Strategy of NIR Dual‐Excitation Upconversion for Ratiometric Intracellular Detection
doi: 10.1002/advs.201901874
Figure Lengend Snippet: a) Optical layout of the purpose‐built NIR dual laser confocal microscope system (DM: dichroic mirror, FM: flip mirror, CCD: charge coupled device). b) UCL spectra collected at 500–600 nm under 808 nm excitation (UCL ex808 ) in probe‐loaded cells. Panels (1)–(4) showed the different cell groups upon addition of NaClO with 0 × 10 −6 , 0.5 × 10 −6 , 1× 10 −6 , or 2 × 10 −6 m , respectively. c) UCL ratio (UCL ex808 /UCL ex980 ) derived from (b) by normalizing the UCL intensities to their corresponding 540 nm emission under 980 nm excitation. RSDs of UCL ex808 and UCL ratio were given in (b) and (c), respectively ( n = 6 cells). d) Plots of UCL ex808 versus the NaClO concentration. e) Plots of UCL ratio versus the NaClO concentration. f) Calibration curve established for NaClO quantification in live cells. The black dots represent the average UCL ratios of different nanoprobe concentration obtained from (e) for establishment of calibration curve. The red dots (1)–(4) represent the average UCL ratio in MCF‐7 cells pre‐treated by 0 × 10 −6 , 0.5 × 10 −6 , 1 × 10 −6 , and 2 × 10 −6 m of NaClO, respectively, for determination of intracellular ClO − .
Article Snippet: Cell imaging was performed on a
Techniques: Microscopy, Derivative Assay, Concentration Assay